Investment Due Diligence Checklist: Complete Guide for Investors in 2025

Inadequate due diligence causes 60% of failed investments, with average losses of $2.4M per missed red flag, according to CB Insights research. Meanwhile, First Round data shows thorough diligence takes 4-8 weeks on average—creating tension between speed and rigor.

Peony streamlines investment diligence: organized data rooms provide complete materials, track which documents reviewers examine, identify concern areas quickly, and maintain audit trails for IC (Investment Committee) decisions. Purpose-built for investor due diligence.

Here's your complete investment due diligence checklist for 2025.

What is Investment Due Diligence?

Definition: Comprehensive process of evaluating investment opportunities to verify facts, assess risks, validate assumptions, and make informed investment decisions.

Core objectives:

  • Verify company claims and metrics
  • Identify material risks and liabilities
  • Validate market opportunity
  • Assess management team
  • Confirm deal terms accuracy
  • Support valuation analysis

Timeline: 2-4 weeks (seed), 4-8 weeks (Series A+), 8-12 weeks (M&A)

Types of Investment Due Diligence

Financial Due Diligence

Focus: Verify financial health and projections accuracy

Key areas:

Historical performance:

  • Revenue recognition policies
  • Margin trends and sustainability
  • Working capital requirements
  • Cash flow patterns
  • Burn rate and runway

Quality of earnings:

  • Recurring vs. one-time revenue
  • Customer concentration risk
  • Revenue retention rates
  • Hidden liabilities
  • Accounting practices

Projections analysis:

  • Assumption reasonableness
  • Sensitivity analysis
  • Downside scenarios
  • Achievability assessment

Red flags:

  • Revenue recognition irregularities
  • Greater than 50% revenue from top 3 customers
  • Declining margins
  • Negative cash flow trends
  • Undisclosed liabilities

Legal Due Diligence

Focus: Identify legal risks and compliance issues

Key areas:

Corporate structure:

  • Clean cap table
  • Proper authorization
  • Board composition
  • Shareholder rights

Contracts:

  • Material agreements review
  • Change of control clauses
  • Key customer contracts
  • Vendor dependencies

IP ownership:

  • Patents and applications
  • Trademark registrations
  • IP assignment agreements
  • Open source compliance

Litigation:

  • Pending lawsuits
  • Threatened claims
  • Settlement history
  • Insurance coverage

Red flags:

  • IP not properly assigned
  • Material litigation pending
  • Customer contract issues
  • Regulatory violations

Operational Due Diligence

Focus: Assess business operations and scalability

Key areas:

Processes:

  • Sales methodology
  • Customer onboarding
  • Service delivery
  • Quality control

Key dependencies:

  • Key person risk
  • Single vendor reliance
  • Technology dependencies
  • Geographic concentration

Scalability:

  • Process documentation
  • Automation level
  • Growth infrastructure
  • Constraint identification

Red flags:

  • Key person dependencies (greater than 50% revenue)
  • Manual, non-scalable processes
  • Critical single vendor
  • Inadequate infrastructure

Market and Commercial Diligence

Focus: Validate market opportunity and competitive position

Key areas:

Market sizing:

  • TAM/SAM/SOM validation
  • Growth rate verification
  • Addressable opportunity
  • Market timing

Competitive landscape:

  • Competitor assessment
  • Differentiation validation
  • Competitive advantages sustainability
  • Win/loss analysis

Customer validation:

  • Reference calls (3-5 customers)
  • Satisfaction surveys
  • Churn analysis
  • Net Promoter Score

Red flags:

  • Market size overstated
  • Weak competitive differentiation
  • High customer dissatisfaction
  • Increasing churn rates

Technical Due Diligence

Focus: Evaluate technology capabilities and risks

Key areas:

Architecture:

  • Scalability assessment
  • Technology stack review
  • Technical debt evaluation
  • Security practices

Development:

  • Code quality
  • Development velocity
  • Product roadmap
  • Engineering team capabilities

Data and security:

  • Data architecture
  • Security measures
  • Privacy compliance
  • Incident history

Red flags:

  • Significant technical debt
  • Security vulnerabilities
  • Scalability limitations
  • Outdated technology stack

Due Diligence Checklist

Financial Documents

Essential:

  • Audited financials (3 years minimum)
  • Monthly management accounts (24+ months)
  • Financial projections (3-5 years)
  • Cap table (current and fully diluted)
  • Revenue by customer/segment
  • Unit economics analysis
  • Cash flow actuals and forecast
  • Bank statements (6 months)

Analysis required:

  • Revenue quality assessment
  • Margin trend analysis
  • Working capital calculation
  • Burn multiple calculation
  • Scenario modeling
  • Comparable company analysis

Legal and Compliance

Corporate:

  • Certificate of incorporation
  • Bylaws/operating agreement
  • Board minutes (all)
  • Shareholder agreements
  • Prior financing documents

Contracts:

  • Top 10 customer contracts
  • Material vendor agreements
  • Partner agreements
  • Real estate leases

IP:

  • Patent portfolio
  • Trademark registrations
  • Employee IP assignments
  • Contractor IP assignments

Litigation:

  • Pending litigation
  • Threatened claims
  • Settlement agreements
  • Insurance policies

Team and Organization

Leadership:

  • Executive bios and resumes
  • Employment agreements
  • Compensation structure
  • Equity holdings

Organization:

  • Current org chart
  • Headcount plan
  • Key employee retention
  • Option pool details

Culture:

  • Employee satisfaction data
  • Turnover statistics
  • Glassdoor reviews
  • Employer brand strength

Commercial and Market

Customer data:

  • Customer list with ARR/revenue
  • Top customer contracts
  • Customer cohort analysis
  • Sales pipeline detail

Market analysis:

  • TAM/SAM/SOM calculation
  • Market research citations
  • Competitive positioning
  • Win/loss data

Reference calls:

  • 3-5 customer references completed
  • Feedback documented
  • Satisfaction validated
  • Concerns identified

Product and Technology

Product:

  • Product demo completed
  • Feature list reviewed
  • Roadmap assessed
  • Technical architecture evaluated

Technology:

  • Code review completed (sample)
  • Security audit reviewed
  • Technical debt assessed
  • Infrastructure evaluated

Due Diligence Process

Week 1-2: Initial Review

Kick-off:

  • Access data room
  • Review provided materials
  • Identify initial questions
  • Assign workstream owners

Quick assessment:

  • Financial health check
  • Legal risk scan
  • Market validation start
  • Customer reference outreach

Deliverable: Initial red flags list, deep-dive priorities

Week 3-4: Deep Dive

Detailed analysis:

  • Financial model review
  • Contract deep-dive
  • Customer calls
  • Competitive assessment
  • Technical evaluation

Management presentations:

  • Finance deep-dive
  • Product roadmap
  • Sales strategy
  • Technology architecture

Deliverable: Comprehensive findings report

Week 5-6: Validation and Reporting

Verification:

  • Cross-check claims
  • Validate assumptions
  • Test scenarios
  • Benchmark performance

IC preparation:

  • Investment memo drafting
  • Risk analysis
  • Valuation justification
  • Terms recommendation

Deliverable: Investment Committee recommendation

Red Flags by Category

Financial Red Flags

Critical (deal-killers):

  • Revenue recognition fraud
  • Fictitious customers
  • Hidden liabilities (greater than 20% of valuation)
  • Insolvency risk

Serious (require adjustment):

  • Customer concentration greater than 50%
  • Declining margins
  • Negative cash flow acceleration
  • Working capital crisis

Legal Red Flags

Critical:

  • IP not owned/assigned
  • Material litigation with significant exposure
  • Regulatory violations
  • Shareholder disputes

Serious:

  • Customer contract issues
  • Employment violations
  • Compliance gaps
  • Undisclosed agreements

Market Red Flags

Critical:

  • Market size significantly overstated
  • No differentiation from competitors
  • Customer dissatisfaction
  • Technology obsolescence

Serious:

  • Slowing market growth
  • Intensifying competition
  • Losing key customers
  • Weak pipeline

Team Red Flags

Critical:

  • Founder departures planned
  • Key employee exodus
  • Management capability gaps
  • Cultural toxicity

Serious:

  • High employee turnover
  • Compensation misalignment
  • Weak bench strength
  • Geographic dispersion challenges

How Peony Streamlines Diligence

Peony accelerates investment due diligence:

Organized materials:

Complete visibility:

  • Track reviewer activity
  • See which documents examined
  • Identify concern areas
  • Monitor diligence progress

Efficient collaboration:

  • Internal team notes
  • Q&A management
  • Workstream coordination
  • Secure sharing

Audit trail:

  • Complete access logs
  • Document IC decisions
  • Compliance documentation
  • Legal defensibility

Result: Complete diligence faster with better documentation.

Conclusion

Investment due diligence requires systematic evaluation across financial, legal, operational, market, and technical dimensions. While shortcuts create risk, overly lengthy processes lose deals to faster competitors. Effective diligence balances thoroughness with efficiency—focusing on material risks while leveraging technology to accelerate.

Platforms like Peony enable more efficient diligence by organizing materials, tracking review progress, facilitating collaboration, and maintaining complete audit trails—helping investors make better decisions faster.

Streamline your investment diligence: Try Peony

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