17 Top Series A Investors in United States for 2025
US Series A rounds averaged $15M in 2025 with median valuations of $40-60M for high-growth companies, according to PitchBook data. Top Series A investors participated in 300+ rounds raising $5B+ in first half of 2025—making targeted outreach critical for Series A founders.
Peony accelerates Series A fundraising: professional data rooms demonstrate scale readiness, track investor engagement on growth metrics, protect sensitive financials, and signal execution quality. Purpose-built for Series A success.
Here are 17 top Series A investors in the United States for 2025.
Top US Series A Investors
| Investor | Focus | Check Size | Notable Portfolio |
|---|---|---|---|
| Wells Fargo Strategic Capital | Fintech, financial services | $10M-$25M | Financial tech |
| Motorola Solutions VC | Enterprise tech, IoT | $5M-$20M | Technology innovation |
| Serena Ventures | Diverse founders, tech | $5M-$15M | Underrepresented founders |
| EPIC Ventures | Enterprise SaaS | $8M-$20M | B2B technology |
| Navigate Ventures | Climate tech, infrastructure | $10M-$25M | Sustainability |
| Amplify Partners | Developer tools, infrastructure | $5M-$15M | Technical founders |
| Mutual Capital Partners | Consumer, tech | $8M-$20M | Innovation-driven |
| G20 Ventures | Technology | $5M-$15M | Emerging tech |
| Aligned Partners | Enterprise tech | $10M-$20M | B2B SaaS |
| Arena Growth Partners | Growth-stage | $15M-$30M | Scaling companies |
Series A Requirements
Typical thresholds:
Revenue:
- $1M-$3M ARR (SaaS)
- $5M-$10M revenue (e-commerce/marketplace)
- Clear path to $100M ARR
Growth:
- 100%+ year-over-year minimum
- Accelerating preferred
- Sustainable trajectory
Metrics:
- Product-market fit proven
- Unit economics positive or path visible
- LTV/CAC ratio greater than 3x
- Gross margins greater than 70% (SaaS)
- Net revenue retention greater than 100%
Team:
- Full executive team in place
- Domain expertise demonstrated
- Execution track record
- Ability to scale
Series A Pitch Requirements
Deck must show:
Scale achieved:
- $1M+ ARR milestone
- Customer count and growth
- Market penetration
- Geographic expansion
Growth trajectory:
- Monthly/quarterly trends
- Acceleration evidence
- Cohort performance
- Retention metrics
Unit economics:
- CAC detailed by channel
- LTV calculation and assumptions
- Payback period (less than 12 months target)
- Gross margin economics
- Path to profitability
Market expansion:
- Total addressable market (TAM greater than $1B)
- Penetration strategy
- Competitive positioning
- Category creation/leadership path
Use of funds:
- Detailed allocation (team, marketing, product)
- Milestone plan (18-24 month runway)
- Hiring roadmap
- Growth investments
Due Diligence Depth
Series A DD vs. Seed:
More comprehensive:
- 4-8 weeks (vs. 2-4 for seed)
- Customer references (5-10 vs. 2-3)
- Deeper financial review
- Technology assessment
- Market validation
- Team assessment
What investors verify:
Revenue quality:
- Customer contracts reviewed (top 20)
- Retention cohorts analyzed
- Expansion patterns examined
- Churn causes investigated
Growth sustainability:
- Sales pipeline quality
- CAC trends by channel
- Marketing ROI
- Scalability of acquisition
Technology:
- Architecture review
- Code quality assessment
- Security practices
- Technical debt level
- Scaling capabilities
Organization:
- Team capability evaluation
- Hiring plan assessment
- Compensation competitiveness
- Culture and retention
How to Reach Series A Investors
Best approaches:
- Portfolio company introductions (highest conversion)
- Customer introductions (if enterprise buyers)
- Fellow founder connections
- Platform partner referrals (AWS, Stripe)
- Accelerator demo days
When ready:
- $1M+ ARR achieved
- 100%+ growth rate
- Full team assembled
- Unit economics clear
- Series A-worthy metrics
Timeline:
- Start outreach at $750k-$1M ARR
- 3-6 month raise process
- Close at $1.5M-$2M+ ARR
How Peony Supports Series A Fundraising
Peony enables professional Series A processes:
Scale demonstration:
- Organize comprehensive metrics
- Customer case studies
- Growth dashboards
- Professional presentation
Investor intelligence:
- Track engagement on unit economics
- See which metrics investors examine
- Identify serious vs. browsing
- Perfect follow-up timing
Execution signal:
- Professional data room organization
- Modern platform choice
- Attention to detail
- Enterprise readiness
Efficient DD:
- Complete materials ready
- Well-organized structure
- Fast investor review
- Accelerated timeline
Result: Series A-quality fundraising infrastructure.
Conclusion
Series A fundraising requires $1M+ ARR, 100%+ growth, proven unit economics, and full team—with more rigorous due diligence than seed stage. Success requires demonstrating scaling capability, sustainable growth, and professional execution through comprehensive materials and metrics.
Peony enables Series A founders to present professionally, track investor engagement, and demonstrate execution quality—supporting successful growth fundraising.
Series A fundraising platform: Try Peony